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. 2015 Jun 17;6:446. doi: 10.3389/fpls.2015.00446

FIGURE 5.

FIGURE 5

Alterations in CW hydroxycinnamates. CW FA (A,D,G,J), FA-dimers (B,E,H,K) and pCA (C,F,I,L) were analyzed from leaf blade (A–C,G–I) or leaf sheath/stem (D–F,J–L) tissues of T1 transgenic Brachypodium empty-vector control lines (173 and 175) and RNAi-RGP1 lines (371, 373, 382, 384, and 393). The hydroxycinnamate chemical linkages were distinguished as ester-linked (A–F) or ether-linked (G–L). All values were normalized with EVC_173 set to 100%. The EVC_173 values (μg hydroxycinnamate mg-1 CW ± SEM) were 6.59 ± 0.13, 1.23 ± 0.11, 3.49 ± 0.14, 6.90 ± 0.04, 0.83 ± 0.07, 6.49 ± 0.09, 1.43 ± 0.05, 1.13 ± 0.04, 0.85 ± 0.18, 4.76 ± 0.10, 1.32 ± 0.04, and 1.43 ± 0.04, respectively for (A–L). Error bars indicate SEM. a,b significantly different from EVC_173 and EVC_175 values, respectively (ANOVA with post hoc Tukey test, alpha = 0.05).