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. 2015 Jun 1;29(11):1120–1135. doi: 10.1101/gad.259119.115

Figure 3.

Figure 3.

path is a permissive factor for dendrite growth. (A,B) Growth defects of path mutants are reversible. path mutant [pathdg50/Df(3L)BSC773] larvae carrying a path rescue transgene (UAS-pathA-GFP) without (left) or with (right) a heat-inducible Gal4 driver (HS-Gal4) were heat-shocked for 1 h at 37°C at the indicated time, and effects on dendrite growth were assessed at 144 h AEL using ppk-CD4-tdTomato to label C4da dendrites. (B) Quantification of C4da dendrite length (in millimeters) in path mutants at 144 h AEL after resupplying UAS-pathA-GFP at the indicated times. n = 8 neurons for each genotype. (C,D) Resupplying path to path mutants induces heterochronic growth. (C) Time-lapse imaging of dendrite growth prior to a 1-h heat shock (72 h AEL; left), 24 h after heat shock (96 h AEL), 48 h after heat shock (120 h AEL), and 72 h after heat shock (144 h AEL) in larvae of the indicated genotype. (D) Plot depicting growth profile of C4da dendrites in the indicated genotypes following induction of path expression. Note that path expression induces rapid late stage growth in path mutants. n = 8 neurons for each genotype. (**) P < 0.01; (***) P < 0.001; (ns) not significant compared with wild-type controls unless otherwise indicated; one-way ANOVA with a post-hoc Dunnett's test. Bars, 50 μm.