Path regulates dendrite growth via effects on translation. (A) Increased translational repression contributes to growth defects of path mutants. Representative images of C4da dendrites are shown for a wild-type control, neuronal expression of UAS-pum or UAS-pum(RNAi), and path mutant larvae expressing UAS-pum(RNAi) in neurons. Quantification of dendrite growth phenotypes is shown for the indicated genotypes. (B) Reduced translational capacity likely contributes to dendrite growth defects in path mutants. RNAi of core translational machinery such as Rpl22 causes growth defects similar to path, whereas overexpression of eIF4e partially mitigates path mutant growth defects. Quantification of dendrite growth phenotypes is shown for the indicated genotypes. (C) path can cooperate with TORC1 to promote dendrite growth. (Left) Representative C4da neurons expressing UAS-pathA-GFP, UAS-Rheb, or both are shown. (Right) Quantification of the effects of UAS-pathA-GFP and/or UAS-Rheb expression on C4da dendrite growth. n ≧ 7 cells for each bar. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; compared with wild-type controls in A and C and path mutants in B; one-way ANOVA with a post-hoc Dunnett's test. Error bars indicate standard deviation. Bars, 50 μm. Dendrite length measurements are in millimeters.