Figure 4. Detection of neuron and microglia cells in the hippocampus of mice by immunohistochemical staining. A-C, Neu-N and Iba-1 immunofluorescence staining in the hippocampus of the control, model, and puerarin groups (10×). D-F, Hippocampal neurons of each group. G-I, Hippocampal microglia of each group. J, Number of neurons in the hippocampus of each group. K, Number of microglia cells in the hippocampus of each group. DG: dentate gyrus. **P<0.01 vs control group; ^#P<0.05, ^##P<0.01 vs model group (one-way ANOVA).