Figure 1. Effects of pulmonary rehabilitation mixture (PRM) on epithelial-mesenchymal transition (EMT) in A549 cells. A, Representative light microscopic appearance of A549 cells for the normal (A1), transforming growth factor (TGF)-β1-treated (A2), and TGF-β1+0.3 µg/mL PRM (A3). B-D, A549 cells were incubated with TGF-β1 (5 ng/mL) for 24 h. E-cadherin, vimentin, high mobility group protein B1 (HMGB1), and receptor for advanced glycation end-product (RAGE) expression were analyzed by Western blot. Results are reported as percent increase over the normal. Data are reported as means±SD. PCNA: proliferating cell nuclear antigen. ^#P<0.01 vs the normal group; *P<0.05 vs the TGF-β1 group (one-way ANOVA followed by Dunnett's test).