Figure 2. Effects of pulmonary rehabilitation mixture (PRM) on epithelial-mesenchymal transition (EMT) in high mobility group protein B1 (HMGB1) small interfering RNA (siRNA) A549 cells. Representative light microscopic appearance of HMGB1 siRNA A549 cells for HMGB1 siRNA A549 (A1), transforming growth factor (TGF)-β1-treated HMGB1 siRNA A549 (A2), and TGF-β1+PRM 0.3 µg/mL HMGB1 siRNA A549 (A3). B-D, HMGB1 siRNA A549 cells were incubated with TGF-β1 (5 ng/mL) for 24 h. E-cadherin, vimentin and HMGB1 expression were analyzed by Western blotting. Results are reported as means±SD percent increase over the normal. PCNA: proliferating cell nuclear antigen. ^#P<0.01 vs the HMGB1 siRNA group (one-way ANOVA followed by Dunnett's test).