A,B (A) Multiplexed strand displacement with increasing amounts of matched Target monitored by UV absorption and (B) differential scanning calorimetry. For (A), All samples contained 0.7 μM each of , and . For (B), All samples contained 20 μM each of , and . For both (A) and (B), the ratios of Target to were 0 (black), 0.5 (red), 1.0 (green), 1.5 (blue), 2.0 (Cyan), and 3.0 (purple).