FIGURE 4.

A,B: Simulations of the fraction of free probe under the conditions of the UV experiments shown in Figure 3a. (A) All samples contained 0.7 μM each of P, ${\mathrm{C}}_6^{\ast }$, ${\mathrm{C}}_{12}^{\ast }$, and ${\mathrm{C}}_{24}^{\ast }$. From left to right, the ratios of T to P were 0, 0.5, 1.0, 1.5, 2.0, and 3.0, which are the same ratios as for the experimental data in Figure 3. (B) Breakdown of the simulated curve for a ratio of 0.5 Target to Probe (+) into contributions from ${\mathrm{C}}_{24}^{\ast }$ (blue curve), ${\mathrm{C}}_{12}^{\ast }$ (red curve), ${\mathrm{C}}_6^{\ast }$ (black curve), and T (green curve).