Microarray analysis of IgE binding to overlapping 20-mer peptides of Ara h 2, left, and Ara h 6, right. Sera are from 4 of 30 representative patients with varying histories regarding the severity of reactivity. From top to bottom: D61, grade I (less severe); D71, grade II; D74 grade III; and D60, grade IV (most severe). Linear peptide regions that bind peanut-specific IgE are colour-coded (see Fig. 2). The Y axis is a calculated z-score (see Methods). The established cut-off score of z = 3 is shown as a horizontal line.