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. Author manuscript; available in PMC: 2015 Jun 17.
Published in final edited form as: Toxicol In Vitro. 2014 Jul 12;28(8):1413–1423. doi: 10.1016/j.tiv.2014.07.002

Fig. 5.

Fig. 5

Hierarchical cluster analysis. (A) RealSkin secretome normalized relative to vehicle and (B) RealSkin co-cultured with MUTZ-LCs secretome normalized relative to vehicle after treatment with sensitizers isoeugenol (IE), p-phenylenediamine (PPD), and irritant, salicylic acid (SA) for 48 hours. No discriminant patterns emerged in the RealSkin mono-culture with sensitizer treatment and therefore the cluster analysis does not arrange the treatments according to their actual potency. However, for the co-culture condition, a panel of cytokines (VEGF, IL-4, IL-12, IL-9) show elevated levels of secretion relative to both irritant and vehicle for sensitizer treatments of moderate and strong potency. Heat map data is represented as mean for N = 3 independent replicates.