Table 1.
Analysis of margin distances identified by support vector machine analysis. The margin distances quantified from the support vector machine ranked, from greatest to least distance of separation, between non-sensitizer treatment groups and sensitized groups for metrics from a Bioplex screen using supernatant from co-culture of RealSkin with MUTZ-3 derived Langerhan’s cells (RSLC). The accuracy, sensitivity, and specificity for each cytokine metric were determined using a support vector machine classification model with 10-fold cross. The top ten secretion metrics (IL-12, IL-9, VEGF, IFN-γ, IL-4, PDGF, IL-8, IL-7, GM-CSF, and IL-6) that can accurately classify non-sensitizers (vehicle and salicylic acid (SA)) from sensitizers (isoeugenol (IE) and p-phenylenediamine (PPD)) identified by SVM all have p values ≤ 0.05 as determined by ANOVA. IL-12 is the only metric that has an accuracy, sensitivity, and specificity value that exceeds 90%. Data analyzed by the SVM included all treatment conditions (untreated, vehicle, SA, IE, and PPD) and all of their respective concentrations for N = 3 independent replicates.
| Rank | Metrics | Margin distance | Accuracy (%) | Sensitivity (%) | Specificity (%) | P-value |
|---|---|---|---|---|---|---|
| 1 | IL-12 | 0.5564 | 91.67 | 91.67 | 91.67 | <.0001 |
| 2 | IL-9 | 0.3390 | 83.33 | 83.33 | 83.33 | 0.00011 |
| 3 | VEGF | 0.3176 | 83.33 | 83.33 | 83.33 | 0.00022 |
| 4 | IFN-γ | 0.2645 | 66.67 | 66.67 | 66.67 | 0.00146 |
| 5 | IL-4 | 0.2564 | 75.00 | 66.67 | 83.33 | 0.00287 |
| 6 | PDGF-bb | 0.2473 | 79.17 | 83.33 | 75.00 | 0.00732 |
| 7 | IL-8 | 0.2438 | 70.83 | 66.67 | 75.00 | 0.01131 |
| 8 | IL-7 | 0.2403 | 66.67 | 50.00 | 83.33 | 0.01559 |
| 9 | GM-CSF | 0.2391 | 75.00 | 58.33 | 91.67 | 0.02965 |
| 10 | IL-6 | 0.2336 | 70.83 | 58.33 | 83.33 | 0.02993 |
| 11 | IL-17 | 0.2278 | 66.67 | 75.00 | 58.33 | 0.05489 |
| 12 | IL-15 | 0.2233 | 58.33 | 66.67 | 0.50 | 0.083 |
| 13 | IL-10 | 0.2219 | 66.67 | 58.33 | 75.00 | 0.09419 |
| 14 | IL-1β | 0.2196 | 62.50 | 50.00 | 75.00 | 0.09501 |
| 15 | FGF basic | 0.2189 | 66.67 | 66.67 | 66.67 | 0.13044 |
| 16 | TNF-α | 0.2181 | 66.67 | 75.00 | 58.33 | 0.15035 |
| 17 | IL-1ra | 0.2160 | 50.00 | 25.00 | 75.00 | 0.16244 |
| 18 | G-CSF | 0.2147 | 50.00 | 25.00 | 75.00 | 0.22958 |
| 19 | IL-2 | 0.2137 | 54.00 | 50.00 | 58.33 | 0.2565 |
| 20 | IP-10 | 0.2117 | 50.00 | 16.67 | 83.33 | 0.2815 |
| 21 | Rantes | 0.2104 | 50.00 | 50.00 | 50.00 | 0.42441 |
| 22 | Eotaxin | 0.2090 | 33.33 | 33.33 | 33.33 | 0.53499 |
| 23 | MIP-1α | 0.2089 | 33.33 | 25.00 | 41.67 | 0.79776 |
| 24 | MIP-1β | 0.2087 | 25.00 | 33.36 | 16.67 | 0.80659 |
| 25 | IL-13 | 0.2087 | 45.83 | 8.33 | 83.33 | 0.86399 |
| 26 | MCP-1 | 0.2087 | 33.33 | 16.67 | 50.00 | 0.89127 |
| 27 | IL-5 | 0.2085 | 45.83 | 50.00 | 41.67 | 0.90373 |