Specifications | |
---|---|
Organism/cell line/tissue | Vibrio cholerae serogroup O1 Ogawa, El Tor biotype |
Sex | Not applicable |
Sequencer or array type | Illumina |
Data format | Raw data: bam files; analyzed data: xls files. |
Experimental factors | Cultures of the wild type bacterium and its isogenic mutant containing a deletion of the gene encoding the histone-like nucleoid structuring protein (H-NS); cultures of the wild type bacterium expressing H-NS tagged with the FLAG epitope. |
Experimental features | Differential RNA-Seq: DNA-free total RNA was extracted from wild type and hns mutant; the samples were depleted of ribosomal RNA and the remaining mRNA was subsequently converted to cDNA. Libraries were prepared using TruSeq technology and standard paired-end sequencing reactions were performed. Chromatin Immunoprecipitation (ChiP)-Seq: DNA from cultures of V. cholerae expressing H-NS-FLAG was immunoprecipitated with the anti-FLAG M2 monoclonal antibody. Libraries for Next Generation Sequencing were prepared using the Illumina TruSeq ChIP kit. Short 50 bp paired-end reads were obtained using the Illumina HiSeq 2000 system. |
Consent | Not applicable |
Sample source location | Morehouse School of Medicine, Atlanta, GA, United States of America. |