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. 2015 Jun 10;5:147–150. doi: 10.1016/j.gdata.2015.05.039
Specifications
Organism/cell line/tissue Vibrio cholerae serogroup O1 Ogawa, El Tor biotype
Sex Not applicable
Sequencer or array type Illumina
Data format Raw data: bam files; analyzed data: xls files.
Experimental factors Cultures of the wild type bacterium and its isogenic mutant containing a deletion of the gene encoding the histone-like nucleoid structuring protein (H-NS); cultures of the wild type bacterium expressing H-NS tagged with the FLAG epitope.
Experimental features Differential RNA-Seq:
DNA-free total RNA was extracted from wild type and hns mutant; the samples were depleted of ribosomal RNA and the remaining mRNA was subsequently converted to cDNA. Libraries were prepared using TruSeq technology and standard paired-end sequencing reactions were performed.
Chromatin Immunoprecipitation (ChiP)-Seq:
DNA from cultures of V. cholerae expressing H-NS-FLAG was immunoprecipitated with the anti-FLAG M2 monoclonal antibody. Libraries for Next Generation Sequencing were prepared using the Illumina TruSeq ChIP kit. Short 50 bp paired-end reads were obtained using the Illumina HiSeq 2000 system.
Consent Not applicable
Sample source location Morehouse School of Medicine, Atlanta, GA, United States of America.