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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Anal Biochem. 2014 Mar 25;456:61–69. doi: 10.1016/j.ab.2014.03.012

Figure 4.

Figure 4

Time course for the formation of acetate. At −25 minutes the assay reagents are mixed and incubated at room temperature (~25°C). Equilibration is complete in <20 minutes, forming ~4 μM NADH. The reaction is initiated (t = 0 min) by addition of 20 μM acetate. The reaction reaches equilibrium at ~30 min and the signal is stable for at least one hour.