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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Anal Biochem. 2014 Mar 25;456:61–69. doi: 10.1016/j.ab.2014.03.012

Figure 5.

Figure 5

Continuous assay to measure HDAC8 activity. The initial rates for deacetylation of the Ac-KGGAKAc-NH2 peptide (100 μM) measured using the acetate assay in a continuous formate are 0.011 ± 0.00041 μM s−1 and 0.022 ± 0.00043 μM s−1 for 0.5 μM (□) and 1 μM (○) Co(II)-HDAC8, respectively. A side-by-side acetate assay using the stopped formate (0.5 μM Co(II)-HDAC8, 100 μM Ac-KGGAKac-NH2) has an initial rate of 0.018 ± 0.0021 μM s−1(■).