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. Author manuscript; available in PMC: 2016 May 19.
Published in final edited form as: Biochemistry. 2015 May 4;54(19):3037–3050. doi: 10.1021/acs.biochem.5b00150

Figure 3. kcat/Km,NAD for SIRT1, SIRT2, SIRT3, and SIRT6 deacylation reactions.

Figure 3

kcat/Km,NAD determined for SIRT1, SIRT2, SIRT3, and SIRT6 in the presence of acetyl-, hexanoyl-, decanoyl-, and myristoyl-lysine H3K9 peptides. Calculated parameters determined from non-linear regression fits to Michaelis-Menten shown below. **kcat/Km,NAD with acetylated peptide for SIRT6 was not measured due to prohibitively high peptide substrate necessary to saturate the reaction (n ≥ 3, ± standard deviation of mean)