. Author manuscript; available in PMC: 2016 May 19.

Published in final edited form as: Biochemistry. 2015 May 4;54(19):3037–3050. doi: 10.1021/acs.biochem.5b00150

Table 1. Rapid-quench rates of nicotinamide and deacylated product formation.

Average rate and standard deviation of two to three independent experiments.

Nicotinamide formeition rate, k₅ (s⁻¹)
	Acetyl	Hexanoyl	Decanoyl	Myristoyl
SIRT2	1.6 ± 3×10⁻¹	2.9×10⁻¹ ± 3×10⁻²	4.3×10⁻¹ ± 6×10⁻²	9×10⁻¹ ± 2×10⁻¹
SIRT3	3.8 ± 6×10⁻¹	2.8×10⁻¹ ± 5×10⁻²	4.0×10⁻¹ ± 4×10⁻²	7.6×10⁻¹ ± 6×10⁻²
SIRT6	N.D.	4.4×10⁻³ ± 6×10⁻⁴	7.3×10⁻² ± 8×10⁻³	1.4×10⁻¹ ± 2×10⁻²

Deacylated peptide formation rate, k₉ (s⁻¹)
	Acetyl	Hexanoyl	Decanoyl	Myristoyl
SIRT2	5.3×10⁻¹ ± 6×10⁻²	1.1×10⁻¹ ± 1×10²	9.0×10⁻² ± 8×10⁻³	5.3×10⁻² ± 5×10⁻³
SIRT3	6.5×10⁻¹ ± 2×10⁻²	8×10⁻² ± 1×10⁻²	1.0×10⁻¹ ± 1×10⁻²	6.5×10⁻² ± 6×10⁻³
SIRT6	N.D.	2.3×10⁻³ ± 1×10⁻⁴	3.3×10⁻² ± 3×10⁻³	4.2×10⁻² ± 4×10⁻³