Figure 2.
Overview of experimental design. In 33 nude mice, subcutaneous human colon cancer xengrafts were randomized into three groups. In group 1 (n=19), two consecutive 3D USMI exams using MBVEGFR2 and MBControl each in random order in the same imaging session were performed to assess reproducibility of the imaging technique (total of 4 injections). Mice in group 2 (n=7) were scanned before and 24 hours after a single dose of bevacizumab; mice in group 3 (n=7) served as control group without anti-angiogenic treatment. After scanning, tumor tissues were harvested for ex vivo analysis.