Table 1. Details of primers used for amplification of full length coding sequence of KGF and KITLG.
Genes | Primer Sequence* | Restriction Site | Amplicon Size (bp) |
---|---|---|---|
KGF | Frd 5’-CCGCTCGAGATGCGCAAATGGATACTGA-3’ | XhoI | 585 |
Rev 5’- CCTATGGCAATAACCTAACCATGGCATG-3’ | NcoI | ||
KITLG | Frd 5’- CGCGGATCCATGAAGAAGACACAAACTT-3’ | BamHI | 825 |
Rev 5’- AGAGTTTCAAGAAGTGTAACTCGAGCGG-3’ | XhoI |
*The restriction sites in the respective primer sets have been italicized and underlined.