Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Cutan Pathol. 2014 Dec 11;42(2):108–117. doi: 10.1111/cup.12444

Figure 4.

Figure 4

TERT promoter and telomere analysis. (A) Sanger sequencing of the TERT promoter sequencing of the primary melanoma reveals a wild type promoter. The metastatic cerebellar lesion carried a C228T mutation in the TERT promoter. (B) Representative nuclei stained for TIF foci using 53BP1 to identify sites of DNA damage (red) and TTAGGG FISH to identify telomeres. Yellow signals indicate co-localization. Dashed lines outline the cell nucleus. Scale bar=2µm. (C) Quantitation of the percentage of cells with ≥4 TIFs per nucleus. Greater than 100 cells were scored for each replicate.