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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Cancer Res. 2015 Apr 21;75(12):2501–2509. doi: 10.1158/0008-5472.CAN-14-3361

Figure 6. In vivo ultrasound molecular imaging.

Figure 6

(A) Representative transverse B-mode and contrast mode ultrasound images following injection of B7-H3-targeted contrast microbubbles show strong signal in breast cancer and only background signal in a mammary gland with normal breast tissue (both outlined by a green region of interest). (B) Photomicrographs of immunofluorescence images (double stained for both the vascular marker CD31 (red) and B7-H3 (green)) confirm expression of B7-H3 on tumor neovasculature (arrows, yellow signal on merged images) in breast cancer with little to no vascular expression in normal tissue. Note B7-H3 is also expressed on tumor epithelium (arrowheads; green). (C) Bar graph summarizes quantitative B7-H3-targeted ultrasound molecular imaging signal obtained in normal and breast cancer in a total of 183 mammary glands with significantly increased imaging signal in breast cancer versus normal tissue. *P<0.001; error bars = standard deviations. (D) Receiving operator characteristic (ROC) curve in distinguishing normal from breast cancer based on quantitative ultrasound molecular imaging signal.