Table 3.

Nonsynonymous substitutions of pTert in 24 PL mice

Substituted position			SC-LL					SC-inbred					CH-RAN		CH-MK		CH-wild

Nucleotide	Amino acid	Motif name^a	1	2	3	4	5	1	2	3	4	5	1	2	1	2	1	2	3	4	5	6	7	8	9	10
182 G/A	Gly61Asp	Motif GQ											+			+									+
182 A/A		Motif GQ	+	+		+	+
695 G/A	^*Arg232Gln												+		+	+
695 A/A														+
733–734 AA/CT	^*Lys245Leu		+	+	+	+	+	+	+	+	+
799 A/G	Thr267Ala																			+	+
962 A/G	Gln321Arg				+	+											+
1115 G/A	Gly372Asp																						+
1279 G/A	Ala427Thr				+		+
1283 C/T	Ser428Leu				+	+
1292 G/A	Ser431Asn	Motif QFP														+
1541 A/G	Asn514Ser				+		+
1652 G/G	^*Thr551Arg	Motif T											+
1690 C/T	^*Arg564Cys	Motif T											+
1757 A/G	Gln586Arg												+	+				+		+	+		+		+	+
1757 G/G			+	+	+	+	+	+	+	+	+	+			+	+
1784 G/A	Arg595Gln	Motif 1													+
2065 C/A	Pro689Thr	Motif A												+
2263 T/G	Ser755Ala					+	+								+
2263 G/G			+	+
2317 G/T	Gly773Cys																				+
2338 G/A	^*Val780Ile												+	+	+		+						+		+	+
2338 A/A								+	+	+	+	+				+
2681 T/C	Met894Thr	Motif D											+	+	+		+		+	+						+
2776 G/A	Asp926Asn	Motif E-1			+	+
2876 A/G	lys959Arg	Motif E-1			+	+																		+
3178 G/A	Ala1060Thr	Motif E-III																		+
3276 G/T	Gln1092His				+		+

		TRF (kb)	4	4.1	5.1	3.9	4.4	16.4	14.8	22.4	22	22	14.9	13.4	12.6	15.3	11.3	18.5	17	16.6	14.9	14.5	14.7	14.8	19.1	17.1

pTert sequence analysis were performed in a total of 24 PL mice: 5, 5, 2, 2, and 10 mice in SC-LL, SC-inbred, CH-RAN, CH-MK, and CH-wild groups, respectively.

Motifs which carried substituted amino acid residues.

These substitutions were occurred at the conserved residues among nine mammalian species. PL mice with substituted residues are marked with “+”. The last row of Table shows TRF lengths (kb) of individual PL mice.

For example, heterozygous and homozygous substitutions are shown like 182 G/A and 182 A/A, respectively.