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Comparison of CE and LC figures of merit for bovine hemoglobin pepsin digest separation
| Characteristic | LC | CE |
|---|---|---|
| Separation speed (min) | 3-12 | 1-4 |
| Peak width (s) | 5-10 | <1 |
| Injection amount (fmol) | ~50000 | ~5 |
| Flow rate (μL/min) | 50-100 | <1 |
| Hb peak capacity/min | 4.4 | 62 |
| Retention/Migration time %RSD |
0.4 | 1.1 |
| Commercial availability | Waters M-Class UPLC with HDX Technology |
None |
| MS modifications required | None | Moderate source changes |
