Skip to main content
. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biol Trace Elem Res. 2015 Apr 21;166(1):72–81. doi: 10.1007/s12011-015-0296-5

Figure 1. Arsenic treatment increases the levels of H3.1 mRNA containing the sequence downstream of the stem-loop sequence.

Figure 1

(A) The nucleotide sequence of the histone H3.1 gene (HIST1H3C). The start codon and stop codon are underlined twice. The stem-loop sequence and potential poly(A) site are boxed. The region transcribed by the stem-loop mechanism is underlined. The primers used for amplifying H3.1 cDNA containing the downstream sequence of the stem-loop sequence are underlined by an arrow. (B) RT-PCR results. Upper panel: BEAS-2B cells were treated with (As) or without (Ctrl) 1 µM arsenic for 48 hours. Total RNA was extracted and then converted to cDNA. H3.1 cDNA with the downstream sequence of the stem-loop sequence was then amplified by PCR with specific primers as shown in (A). g-tubulin was used as an internal control. Lower panel: The bar graphs show relative quantification of H3.1 mRNA levels normalized to g-tubulin. The data shown are the means ± S.D. from experiments performed in triplicate. * p<0.05.