Position of BRAF mutation and conservation across species. A) Multiple sequence alignment of the BRAF protein sequences from eight species including dog, human, chimpanzee, mouse, rat, cat, zebrafish, and chicken. Colors indicate level of conservation with black being no conservation and maroon being a perfect match. Alignment and conservation scoring were performed with the PRALINE multiple sequence alignment tool (http://www.ibi.vu.nl/programs/pralinewww/). The star highlights the canonical mutation. B) Frequency of somatic BRAF mutations by protein position among human tumors in the COSMIC database (34).