(A) 786-O (VHL-mut) and Caki-1 (VHL-wt) cells were treated with media at the indicated glucose concentration, or with the indicated concentration of 2-DG in 5 mM glucose media, for 72 hours and then subjected to MTT assays. *P < 0.05 compared to 5 mM glucose or 0 mM 2-DG. Error bars indicate standard deviation. Data are representative of three repeats.
(B) 786-O (VHL-mut) and Caki-1 (VHL-wt) cells were treated with glucose depleted media (Glc-) or 2-DG (5 mM) for 24 hours then lactate in the media was measured as described in Materials and Methods. *P < 0.05 compared to control (glucose 5 mM). Error bars indicate standard deviation. Data are representative of three repeats.