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Throughput and sensitivity of the sorter
| Genotype |
us (μm/s) |
u3 (μm/s) |
Total number of worms |
% in initial mix |
Sorting time (h) |
% in holding chamber |
% in collection chamber |
Throughput (#/h) |
|---|---|---|---|---|---|---|---|---|
| unc-63 | 40 | 165 | 496 | 50 | 1.6 | 90.9 | 0.7 | 317 |
| dys-1 | 160 | 204 | 991 | 50 | 1.1 | 90.0 | 11.7 | 901 |
| lev-10 | 121 | 127 | 1036 | 50 | 0.4 | 96.0 | 10.7 | 2486 |
| Average | 92.3 | 7.7 | 1235 |
The % are for the slow mutants, which lacked fluorescent protein expression.
us: Swimming velocityu3: Fluid velocity in the separation conduit
