Figure 7.

YAP/TAZ regulate the transcription of genes important for cell cycle progression and survival. (A) SCC2 cells were transiently transfected with control siRNA (siCTL) or siRNA targeting TAZ (siTAZ), YAP (siYAP), or YAP and TAZ (siYAP/TAZ). Relative expression of genes indicated in the microarray analysis was determined by qPCR. All data are relative to siCTL (dashed line) and are shown as the average of three experiments (+S.E.). Genes are grouped together by function including knockdown efficiency (blue), cell cycle regulation (red), pro-survival (green), and transcription factors (purple). (B) Doxycycline-inducible CAL27 control cells or cells expressing 3xFLAG-YAP(5SA) or 3xFLAG-YAP(5SA/S94A) were treated with doxycycline. Relative expression of genes indicated in the microarray analysis was determined by qPCR. All data are relative to control cells (dashed line) and are shown as the average of three experiments (+S.E.). Genes are grouped together by function including cell cycle regulation (red), pro-survival (green), and transcription factors (purple). Statistics comparing to the control cells (dashed line) were calculated using an unpaired Student’s t test and are represented as * p-value<0.05, ** p-value<0.01, and *** p-value<0.001. (C) Box plot of TEAD4 expression with respect to OSCC tumor grade and stage, showing TEAD4 expression is significantly induced with OSCC tumor grade (One-way ANOVA; p-value=3.51 e-05) and stage (One-way ANOVA; p-value=0.0012). Pairwise t-tests of expression between groups is also shown, and represented as * p-value<0.05, ** p-value<0.01, and *** p-value<0.001.