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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Curr Biol. 2015 May 28;25(12):1573–1582. doi: 10.1016/j.cub.2015.04.038

Figure 5. In the presence of the phosphate mimic, BeF3, Tm1A binding shifts to the barbed end, and becomes transient.

Figure 5

(A–B) Visual representation of each binding event of 180nM Cy5-Tm1A to actin filaments formed in ATP (A), or in BeF3 (B). Each filament’s length is plotted as a black bar, and the distance from the pointed end of each binding event is plotted in red. (C) Binding of Tm1A to BeF3-bound actin filaments is transient. Representative hand-drawn kymographs of A488-actin and Cy5-Tm1A, with and without BeF3. White scale bar: 5μm. Black time bar: 500 sec. (D) Visual representation of each binding event of 450nM Cy5-Tm1A to ATP-actin filaments (left) or ADP-actin filaments (right). (See also Figure S5).