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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Magn Reson Med. 2014 Dec 17;74(6):1750–1757. doi: 10.1002/mrm.25566

Figure 2. Engineering subcellular localization of MntR.

Figure 2

(a) The schematic shows the different MntR localization tags and subcellular locations being targeted. The tags employed included an N-terminal signal sequence (SS) for entry into the secretory pathway, a C-terminal amino acid KDEL sequence for retention in the Endoplasmic Reticulum (ER), and a fusion of MntR with Cab45 for localization within the Golgi Apparatus (GA). (b) Immunohistochemistry was performed on HEK cells expressing the MntR localization chimeras, co-stained with subcellular markers. Without engineering MntR expressed in the cytosol, similar to GFP. With the addition of the SS and KDEL sequences, MntR entered the secretory pathway and was localized in the ER. Fusion with Cab45 also brought MntR into the secretory pathway, but was localized more distally, in the GA. The CabMntR fusion localization was indistinguishable from CabMntRKDEL by immunostaining, since both colocalized with the GA marker.