Fig 4. Assessment of T. cruzi msh2 knockout mutant infectivity in vitro.

(A) T. cruzi trypomastigote cells released by Vero cells infected with either WT or with three cloned cell lines of Tcmsh2-/- mutants were counted and equal numbers were used to infect Vero cells attached to glass coverslips or (B) cultured intraperitoneal macrophages extracted from BALB/c mice. Graphs show the average number of intracellular parasites counted per 100 cells; vertical lines denote standard deviation. **p<0.01, *p<0.05: one-way ANOVA with Bonferroni post-test of knockout mutants relative to wild type.