Skip to main content
. 2015 Jun 17;10(6):e0128443. doi: 10.1371/journal.pone.0128443

Fig 4. Light induced transcription via light mediated nuclear translocation in yeast.

Fig 4

(A) NMY51 contains his3, ade2 and lacZ genomic reporter genes under the control of LexAop. (B) Schematic of the LANS controlled artificial transcription factor in yeast (C) Growth assay of LANS controlled transcription factor in NMY51. The left panel shows growth on media lacking leucine, which confers plasmid resistance and demonstrates that the light used does not affect regular yeast growth. The right panel demonstrates light dependent growth on media lacking leucine, histidine and adenine. (D) β-galactosidase activity measurements upon blue light induced transcription activation with LANS4 n = 3 each, mean reported ± SEM and statistical significance is calculated with unpaired two-tailed t-student’s test (p = 0.0019).