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Validation of pTyr363-Ub interactions. (a) Non-reduced pulse-chase lysine discharge reactions showing the disappearance of UbcH5B~Ub in the presence of CBL-BLRR or pCBL-BLRR over time. (b-f) 31P-NMR chemical shift perturbation data for pCBL-BLRR (b) alone, (c) with UbcH5B, (d) with UbcH5B–Ub, (e) with UbcH5B–Ub T9A, and (f) with UbcH5B–Ub K11A.
