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Effect of pTyr363 on the kinetics of Ub transfer. (a) Kinetics of diUb formation catalyzed by CBL-BLRR. The rate of diUb formation was plotted against UbcH5B concentration for wild type [32P]Ub. (b) As in a but performed with pCBL-BLRR. (c) As in b but with [32P]Ub T9A (d) As in b but with pCBL-BLRR K381A. Kinetic parameters were determined from three independent datasets (n = 3). Error bars, s.d.
