Fig. 3.

ROS production in Nrf2-KO, Keap1-KD and wild-type (WT) neuronal co-cultures and organotypic slice cultures. ROS production is significantly higher in Nrf2-KO both in glio-neuronal co-culture (A and B; WT: n = 49 cells; 3 experiments; Nrf2-KO: n = 70 cells; 3 experiments; Keap1-KD n = 51 cells; 3 experiments) and organotypic slice cultures (C and D WT: n = 72 cells; 3 experiments; Nrf2-KO: n = 62 cells; 3 experiments; Keap1-KD n = 80 cells; 3 experiments). Panel A and C show representative experiments (mean ± SEM) demonstrating increase in ROS as measured by HEt fluorescence over time for the different groups. B and D summarize normalized ROS production rates in histograms. **p < 0.01; ***p < 0.001; ns: not significant.