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. 2015 Apr;1850(4):794–801. doi: 10.1016/j.bbagen.2014.11.021

Fig. 6.

Fig. 6

Mitochondrial ROS production in Nrf2-KO, Keap1-KD and wild-type (WT) glio-neuronal co-cultures. Mitochondrial ROS production was measured with MitoSOX. Traces A –F show mean MitoSOX fluorescence of representative experiments in Nrf2-KO (n = 42), Keap1-KD (n = 46) and WT (n = 32) neurons. Grey background indicates treatment with rotenone (5 μM; A–C; WT (n = 16); Nrf2-KO (n = 20) and Keap1-KD (n = 20) or pyruvate (5 mM; D-F WT (n = 16); Nrf2-KO (n = 22) and Keap1-KD (n = 26). Note that treatment with pyruvate decreases mitochondrial ROS production in Nrf2-KO neurons (F). Histograms summarizing basal mitochondrial membrane potential (G) and basal mitochondrial ROS production (H) in Nrf2-KO, Keap1-KD and WT neurons. The histogram in I shows rates of mitochondrial ROS production in Nrf2-KO, Keap1-KD and WT neurons after treatment with rotenone or pyruvate. Significance levels are indicated above the bars and refer to a comparison between baseline and pyruvate or rotenone. **p < 0.01; *p < 0.05.