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. Author manuscript; available in PMC: 2015 Dec 11.
Published in final edited form as: Nature. 2015 Jun 3;522(7555):236–239. doi: 10.1038/nature14503

Extended Data Figure 7. ESCRT-depletion impairs NE-rim formation.

Extended Data Figure 7

A,B. Timelapse microscopy analysis and quantification of NE-rim formation in HeLa cells stably expressing YFP-LAP2β and mCh-H2B and treated with the indicated siRNA Scale bar is 10 μm. Time for rim formation post anaphase onset given (mins) (Ctrl 8.53 ± 0.09, 226 cells analysed over 8 independent experiments; CHMP2A-1, 7.60 ± 0.09, 205 cells analysed over 7 independent experiments; CHMP2A-2, 6.86 ± 0.12, 37 cells analysed over 2 independent experiments; CHMP2B, 6.92 ± 0.09, 79 cells analysed over 4 independent experiments; CHMP2A and CHMP2B, 6.84 ± 0.13, 50 cells analysed over 2 independent experiments; CHMP4B, 7.07 ± 0.14, 44 cells analysed over 2 independent experiments; UFD1 9.2 ± 0.18, 39 cells analysed over 3 independent experiments. All times mean ± S.E.M, in minutes, images representative of the indicated number of cell analysed). C. Resolved cell lysates from A were analysed by western-blotting with the indicated antisera.