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. Author manuscript; available in PMC: 2015 Dec 11.
Published in final edited form as: Nature. 2015 Jun 3;522(7555):236–239. doi: 10.1038/nature14503

Figure 3. UFD1 directs NE-localisation of CHMP2A.

Figure 3

A. β-galactosidase activity of yeast co-transformed with the indicated Gal4 and VP16 fused proteins (n = 3 ± S.D.). B. MST experiments displaying interaction of HIS-UFD1 with CHMP2A (Fraction unbound displayed, n = 5 ± S.D.) C, D. Immunofluorescence (D) and quantification of NE localisation (C: Control, 35 cells; UFD1-1, 42 cells; UFD1-2, 25 cells; UFD1-3, 39 cells; average ± S.D. presented from 4 independent experiments) in HeLa cells transfected with the indicated siRNA and stained with anti-CHMP2A, anti-tubulin and DAPI, scale bar is 10 μm, images representative of 23 cells (Control), 34 cells (UFD1-1) 14 cells (UFD1-2) and 24 cells (UFD1-3).