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. 2015 Jun 17;10(6):e0129704. doi: 10.1371/journal.pone.0129704

Fig 2. Overexpression of POSTN, COL-1, and FN in the stroma of wound metastasis in a melanoma patient.

Fig 2

(A) Quantitative RT-PCR analysis of POSTN, COL1A1, and FN1 mRNA expression in the primary (n = 3) and metastatic (n = 2) lesions. Data are means ± SD for triplicate samples from one of three representative experiments. *P < 0.05, **P < 0.005. (B) Immunohistochemical staining of the primary and metastatic lesions with antibodies to Melan-A, POSTN, COL-I, and FN. Dashed lines indicate the basement membrane zone. (C) Immunofluorescence analysis of the lower dermis of the metastatic lesion with antibodies to Melan-A (green) as well as those to POSTN, COL-I, or FN (red). FN showed colocalization (yellow) with Melan-A (melanoma cell marker), whereas POSTN and COL-I were localized only in the stroma. (B, C) Bars = 100 μm.