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. 2015 Jun 17;10(6):e0129704. doi: 10.1371/journal.pone.0129704

Fig 6. Inhibition of POSTN expression in microenvironment reduces incidence of melanoma wound metastasis.

Fig 6

(A) Experimental protocol for in vivo assay of metastasis. B16-BL6 cells were transplanted into the left hind footpad of nude mice on day 0, and MC3T3-E1 cells stably expressing control or POSTN shRNAs were injected subcutaneously into the left thigh on day 7. (B, C) Representative images (yellow circle indicates subcutaneous metastasis) of (B) and longest diameter of subcutaneous metastasis at (C) the left thigh on day 23 for mice (n = 7 each) treated as in (A). Quantitative data are presented as a box-and-whisker plot. **P < 0.005. (D) Representative immunohistochemical staining of S100 and POSTN as well as H&E staining at day 23 for the left thigh of mice treated as in (A). Arrowheads indicate POSTN expression in the region surrounding a melanoma tumor cell nest. Dashed lines indicate the periphery of tumor cell nest. Bars = 100 μm.