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. 2015 Jun 17;11(6):e1004957. doi: 10.1371/journal.ppat.1004957

Table 1. N-terminal mutants of pUL31.

Protein Protein sequence BAC pHSV1(17+) Growth
pUL31 7HRRGSR(x)8RRRSR(x)11RRASRKSL44 Lox-UL31 +++
pUL31ΔN M_45PPHARKQELC55 Lox-UL31ΔN -
pSV40NLS-UL31ΔN M_PKKKRKV_M45PPHARKQELC55 Lox-SV40NLS-UL31ΔN -
pUL31-mp1 7HRRGSR(x)8 NAASR (x)11RRASRKSL44 Lox-UL31-mp1 ++
pUL31-mp2 7HRRGSR(x)8 RRRSR (x)11NAASAKSL44 Lox-UL31-mp2 ++
pUL31-mp1mp2 7HRRGSR(x)8NAASR (x)11NAASAKSL44 Lox-UL31-mp1mp2 -
pUL31 7HRRGSR(x)8RRRSR (x)11RRASRKSL44 Lox-UL31-mp1mp2 rescue +++
pUL31 7HRRGSR(x)8RRRSR (x)11RRASRKSL44 Lox-UL31-mp1mp2 rev +++
pSV40NLS-UL31-mp1mp2 M_PKKKRKV_ 2YDTDPHRRGSR(x)8NAASR (x)11NAASAKSL44 Lox-SV40NLS-UL31-mp1mp2 -
pSV40NLS-UL31 M_PKKKRKV_2YDTDPHRRGSR(x)8RRRSR (x)11RRASRKSL44 Lox-SV40NLS-UL31-mp1mp2 rev +++
pUL31-hbp1mp1mp2 7HRRRSR(x)8 NAASR (x)11NAASAKSL44 Lox-UL31-hbp1mp1mp2 +