Table 4. BAC mutagenesis.

Nr.	BAC	Inserted Fragment / Primer / Template	Recipient BAC
1	pHSV1(17+)Lox	-
2	pHSV1(17+)Lox- ΔUL31/galk	galK-kan in UL31 locus (bp 9–865) (PCR: Oligos Nr.1/2; Table 3, Template pGPS-galK-kan)	1
3	pHSV1(17+)Lox—UL31wt (rescue)	UL31 wt (PCR: Oligos Nr.4/5; Table 3; Template pHSV-1(17+)Lox)	2
4	pHSV1(17+)Lox—UL31ΔN	UL31ΔN (PCR: Oligos 4/9; Table 3; Template Lox)	2
5	pHSV1(17+)Lox—SV40NLS-UL31ΔN	SV40NLS-UL31ΔN (PCR: Oligos 4/10; Table 3; Template Lox	2
6	pHSV1(17+)Lox—UL31-mp1mp2	UL31-mp1mp2 (PCR: Oligos Nr.4/5; Table 3; Template pDONR207-UL31-mp1mp2)	2
7	pHSV1(17+)Lox—SV40NLS-UL31-mp1mp2	SV40NLS-UL31-mp1mp2 (PCR: Oligos Nr.4/6; Table 3; Template pCR3-N-c-myc-SV40NLS-UL31-mp1mp2)	2
8	pHSV1(17+)Lox- ΔUL31-mp1mp2/galk	galK-kan in UL31-mp1mp2 locus (PCR: Oligos Nr.1/2; Table 3; Template pGPS-galK-kan)	6
9	pHSV1(17+)Lox—UL31-mp1mp2 rev	UL31 wt (PCR: Oligos Nr.4/5; Table 3; Template pHSV-1(17+)Lox)	8
10	pHSV1(17+)Lox- ΔSV40NLS-UL31-mp1mp2/galk	galK-kan in SV40NLS-UL31-mp1mp2 locus (PCR: Oligos Nr.1/3; Table 3; Template pGPS-galK-kan)	7
11	pHSV1(17+)Lox- SV40NLS-UL31-mp1mp2 rev	UL31 wt (PCR: Oligos Nr.4/7; Table 3; Template pHSV-1(17+)Lox)	10
12	pHSV1(17+)Lox—UL31-mp1	UL31-mp1 (PCR: Oligos Nr.4/5; Table 3; Template pDONR207-UL31-mp1)	2
13	pHSV1(17+)Lox—UL31-mp2	UL31-mp2 (PCR: Oligos Nr.4/5; Table 3; Template pDONR207-UL31-mp2)	2
14	pHSV1(17+)Lox—UL31-hbpmp1mp2	UL31-hbpmp1mp2 (PCR: Oligos Nr. Nr.4/8; Table 3; Template pDONR207-UL31-hbpmp1mp2)	2