Figure 3.

DNA methylation patterns in neural stem cell models of gene dosage disorders are more characteristic of differentiating cells than proliferating cells. (a) Total number of CpG clusters detected and the total number of genome-wide significant CpG clusters that show differential methylation. (b) Graphical representation of the likelihood of observing overlapping CpG clusters in the MBD5 KD, (c) SATB2 KD and (d) cell state experiment. (e–g) Significant GO terms associated with CpG clusters near genes for the cell state (e), MBD5 KD (f) and SATB2 KD (g) experiments. (h–j) Traces showing methylation differences for the most significantly differentially methylated CpG clusters in (h) MBD5 KD, (i) SATB2 KD and (j) most significant CpG clusters that overlap in both SATB2 KD and MBD5 KD. GO, gene ontology; KD, knockdown; MBD, methyl-CpG binding domain; SATB, special AT-rich binding protein.