. 2015 Mar 11;51(3):350–359. doi: 10.1007/s11627-015-9674-0

Table 1.

Media used for the culture of Gentiana protoplasts, callus proliferation, and plant regeneration

Medium code	Medium components
PCM1	MS salts (without NH₄NO₃) and vitamins, 30 g l⁻¹ glucose, 3.0 g l⁻¹ glutamine, 2.0 mg l⁻¹ NAA, 0.1 mg l⁻¹ TDZ^a
PCM2	MS salts (without NH₄NO₃) and vitamins, 30 g l⁻¹ glucose, 3.0 g l⁻¹ glutamine, 2.0 mg l⁻¹ NAA, 1.0 mg l⁻¹ BAP^a
CPM1	MS salts and vitamins, 30 g l⁻¹ sucrose, 2.0 mg l⁻¹ NAA, 0.2 mg l⁻¹ TDZ
CPM2	MS salts and vitamins, 30 g l⁻¹ sucrose, 2.0 mg l⁻¹ NAA, 1.0 mg l⁻¹ BAP
CPM3	MS salts and vitamins, 30 g l⁻¹ sucrose, 1.0 mg l⁻¹ dicamba, 0.1 mg/l⁻¹ NAA, 2.0 mg l⁻¹ BAP, 80 mg l⁻¹ adenine sulfate
CPM4	MS salts and vitamins, 30 g l⁻¹ sucrose, 0.5 mg l⁻¹ 2,4-D, 1.0 mg l⁻¹ kinetin
PRM1	MS salts and vitamins, 30 g l⁻¹ sucrose, 20 ml l⁻¹ coconut water, 0.1 mg l⁻¹ NAA, 8.0 mg l⁻¹ TDZ
PRM2	MS salts and vitamins, 30 g l⁻¹ sucrose, 20 ml l⁻¹ coconut water, 0.1 mg l⁻¹ NAA, 6.0 mg l⁻¹ BAP
PRM3	MS salts and vitamins, 30 g l⁻¹ sucrose, 20 ml l⁻¹ coconut water, 1.0 mg l⁻¹ kinetin, 0.5 mg l⁻¹ GA₃, 80 mg l⁻¹ adenine sulfate
PRM4	MS salts and vitamins, 30 g l⁻¹ sucrose, 20 ml l⁻¹ coconut water, 1.0 mg l⁻¹ NAA, 2.0 mg l⁻¹ BAP, 3.0 mg l⁻¹ zeatin, 1.0 mg l⁻¹ GA₃

PCM protoplast culture medium, CPM callus proliferation medium, PRM plant regeneration medium

^aPCM media were supplemented with different concentrations of mannitol (0.5, 0.33, 0.17, or 0.0 M), as described in the “Materials and Methods”