Fig 2. Specificity and cross reactivity of ATX isoform specific mAbs.

(A) Purified hisATXβ and hisATXδ recombinant proteins (200 ng/lane) expressed using the baculovirus system were subjected to a western blot analysis with anti-classical ATX mAb (R4+4 mAb, left) and anti-novel ATX mAb (R4-127 mAb, right). (B) Human, mouse, rat, rabbit, dog and bovine serum (1 μL/lane) were subjected to a western blot analysis with anti-classical ATX mAb (R4+4 mAb, left) and anti-novel ATX mAb (R4-127 mAb, right).