Table 1.
P. lutzii more abundant proteins after incubation with argentilactone.
| Functional categorya | Protein description | Accession numberb | Protein score | Fold change |
|---|---|---|---|---|
| METABOLISM | ||||
| Amino acid metabolism | 1-pyrroline-5-carboxylate dehydrogenase | PAAG_05253 | 1803.48 | 1.768 |
| 4-aminobutyrate aminotransferase | PAAG_00468 | 1503.69 | 1.804 | |
| Homogentisate 1,2-dioxygenase | PAAG_08164 | 739.59 | 1.878 | |
| Methylmalonate-semialdehyde dehydrogenase | PAAG_07036 | 1184.68 | 1.336 | |
| O-acetylhomoserine (Thiol)-lyase | PAAG_08100 | 3986.80 | 1.323 | |
| Serine hydroxymethyltransferase | PAAG_08512 | 1347.52 | 1.221 | |
| Pyruvate decarboxylase | PAAG_02050 | 1361.75 | 1.323 | |
| Sulfite oxidase | PAAG_07811 | 1481.90 | 1.221 | |
| Aminopeptidase B | PAAG_09004 | 450.66 | * | |
| Aspartyl aminopeptidase | PAAG_04205 | 526.39 | * | |
| Aspartyl aminopeptidase | PAAG_00664 | 568.78 | * | |
| Cysteine synthase | PAAG_07813 | 412.94 | * | |
| Hydroxymethylglutaryl-CoA lyase | PAAG_06215 | 1087.40 | * | |
| Formate dehydrogenase-III | PAAG_03599 | 1012.38 | * | |
| Carbohydrate metabolism | Triosephosphate isomerase | PAAG_02585 | 10825.00 | 1.246 |
| Pyruvate dehydrogenase complex component Pdx1 | PAAG_00666 | 997.13 | 1.768 | |
| Pyruvate dehydrogenase complex | PAAG_00050 | 877.32 | 1.616 | |
| Fumarate reductase Osm1 | PAAG_04851 | 2013.07 | 1.234 | |
| 4-hydroxyphenylpyruvate dioxygenase | PAAG_07875 | 4971.33 | 1.568 | |
| N-acetylglucosamine-phosphate mutase | PAAG_01931 | 398.11 | * | |
| Aldehyde dehydrogenase | PAAG_05392 | 399.42 | * | |
| Fumarylacetoacetase | PAAG_08163 | 2404.04 | 1.234 | |
| Nitrogen metabolism | Formamidase | PAAG_03333 | 1620.07 | 1.209 |
| Nucleotide metabolism | Rad4 family protein | PAAG_05019 | 2058.26 | 1.377 |
| Coenzyme metabolism | Riboflavin synthase subunit alpha | PAAG_01934 | 554.30 | * |
| Cell rescue, defense and virulence | Proteasome component C5 | PAAG_00866 | 1414.16 | * |
| Superoxide dismutase [Cu-Zn] | PAAG_04164 | 2348.64 | 1.297 | |
| Sulfur metabolite repression control protein C | PAAG_07339 | 4835.50 | * | |
| ENERGY | ||||
| Eletron transport | Cytochrome c oxidase polypeptide VI | PAAG_07246 | 2376.00 | 1.477 |
| Cytochrome c oxidase polypeptide IV | PAAG_06796 | 711.90 | ||
| Associate energy conservation | Cytochrome c PriAC=F2TJX0 | PAAG_06268 | 1307.21 | 1.522 |
| Glycolysis and gluconeogenesis | 6-phosphogluconolactonase | PAAG_05621 | 688.50 | 1.297 |
| Glyoxylate cycle | Malate synthase | PAAG_04542 | 617.40 | * |
| Krebs cycle | Succinyl-CoA: | PAAG_05093 | 770.38 | * |
| Methyl citrate cycle | 2-methylcitrate dehydratase | PAAG_04559 | 20407.15 | 1.297 |
| Oxidation of fatty acids | Enoyl-CoA hydratase | PAAG_06309 | 3244.11 | 1.716 |
| Acetyl-CoA acetyltransferase | PAAG_03447 | 1578.04 | * | |
| Peroxisomal 3-ketoacyl-coA thiolase | PAAG_03689 | 1248.76 | * | |
| Siderophore-iron transport | Siderophore peptide synthase | PAAG_02354 | 1582.68 | * |
| Protein fate | Chaperone DnaK | PAAG_01339 | 14261.80 | 1.259 |
| Chaperonin | PAAG_05142 | 71219.03 | 1.584 | |
| Chaperonin GroL | PAAG_08059 | 36257.80 | 1.336 | |
| GrpE protein homolog | PAAG_06255 | 6685.85 | 1.649 | |
| Glutathione S-transferase | PAAG_08162 | 766.51 | 1.405 | |
| Peptidylprolyl isomerase | PAAG_05788 | 3381.68 | 1.284 | |
| CORD and CS domain-containing protein | PAAG_02973 | 1899.14 | * | |
| Miscellaneous | Thiol methyltransferase | PAAG_06955 | 1027.93 | 1.391 |
| Translation | Endoribonuclease L-PSP | PAAG_08313 | 12115.91 | 1.234 |
| Unclassified | Uncharacterized protein | PAAG_00297 | 870.53 | 1.649 |
| Uncharacterized protein | PAAG_07772 | 1786.94 | 1.209 | |
a
Functional category—based on the MIPS Functional categories database and GO.
b
Accession number—accession number of matched protein from Paracoccidioides database (http://www.broadinstitute.org/annotation/genome/paracoccidioides_brasiliensis/MultiHome.html).
*
Proteins detected only during incubation with argentilactone.