Table 2.
P. lutzii less abundant proteins after incubation with argentilactone.
Functional categorya | Protein description | Accession numberb | Protein score | Fold change |
---|---|---|---|---|
METABOLISM | ||||
Amino acid metabolism | Acetolactate synthase | PAAG_00221 | 849.43 | 0.726 |
Argininosuccinate synthase | PAAG_07114 | 6934.38 | 0.522 | |
Cobalamin-independent methionine synthase MetH/D | PAAG_07626 | 2518.10 | 0.577 | |
Isovaleryl-CoA dehydrogenase, mitochondrial | PAAG_04102 | 953.65 | 0.811 | |
NADP-specific glutamate dehydrogenase | PAAG_07689 | 1723.70 | 0.600 | |
Ornithine aminotransferase | PAAG_06431 | 1262.54 | 0.684 | |
Lysine decarboxylase-like protein | PAAG_03537 | 800.11 | * | |
NAD-specific glutamate dehydrogenase | PAAG_01002 | 1969.33 | * | |
Saccharopine dehydrogenase | PAAG_02693 | 1249.65 | * | |
Serine hydroxymethyltransferase | PAAG_07412 | 4659.48 | 0.677 | |
Carbohydrate metabolism | Mannitol-1-phosphate dehydrogenase | PAAG_06473 | 4920.79 | 0.726 |
Eukaryotic phosphomannomutase | PAAG_00889 | 1400.74 | 0.691 | |
GDP-mannose pyrophosphorylase A | PAAG_08174 | 860.91 | * | |
Transketolase TktA | PAAG_04444 | 2581.21 | 0.763 | |
Coenzyme metabolism | Adenosylhomocysteinase | PAAG_02859 | 14585.17 | 0.440 |
Dihydropteroate synthase | PAAG_01324 | 870.83 | 0.779 | |
Pyridoxine biosynthesis protein pyroA | PAAG_07321 | 2354.97 | 0.787 | |
S-adenosylmethionine synthase | PAAG_02901 | 6069.65 | 0.357 | |
Nucleotide metabolism | Bifunctional purine biosynthesis protein ADE17 | PAAG_00731 | 4517.89 | 0.811 |
Adenylosuccinate lyase | PAAG_04974 | 686.76 | * | |
S-methyl-5-thioadenosine phosphorylase | PAAG_01302 | 1274.52 | * | |
UDP-N-acetylglucosamine pyrophosphorylase | PAAG_06885 | 768.34 | 0.779 | |
Phosphate metabolism | Inorganic pyrophosphatase | PAAG_00657 | 4020.00 | 0.771 |
Cell cycle and dna processing | Cell division cycle protein 48 | PAAG_05518 | 1782.70 | 0.719 |
D-tyrosyl-tRNA(Tyr) deacylase | PAAG_03334 | 22078.91 | 0.741 | |
Nascent polypeptide-associated complex subunit alpha | PAAG_04571 | 4281.41 | 0.779 | |
Peptidyl-prolyl cis-trans isomerase | PAAG_06168 | 2417.21 | 0.795 | |
Proliferating cell nuclear antigen | PAAG_00923 | 5676.48 | 0.748 | |
TCTP family protein | PAAG_09083 | 23693.70 | 0.463 | |
Thioredoxin | PAAG_02364 | 25560.74 | 0.719 | |
UV excision repair protein Rad23 | PAAG_04949 | 1953.93 | 0.651 | |
Cell rescue, defense and virulence | Heat shock protein 30 | PAAG_00871 | 6591.33 | 0.492 |
Heat shock protein 88 | PAAG_07750 | 15855.80 | 0.811 | |
Heat shock protein SSB | PAAG_07775 | 5550.62 | 0.487 | |
ENERGY | ||||
Eletron transport and membran associate energy conservation | ATP synthase D chain, mitochondrial | PAAG_04570 | 1983.65 | 0.748 |
ATP synthase gamma chain | PAAG_05576 | 4554.87 | 0.595 | |
ATP synthase subunit alpha | PAAG_04820 | 17850.35 | 0.670 | |
ATP synthase subunit beta | PAAG_08037 | 19311.82 | 0.726 | |
Glycolysis and gluconeogenesis | Phosphoenolpyruvate carboxykinase AcuF | PAAG_08203 | 2953.75 | 0.554 |
Pyruvate dehydrogenase E1 component alpha subunit | PAAG_08295 | 904.41 | 0.748 | |
Glucokinase glkA | PAAG_06172 | 746.76 | * | |
Phosphoglucomutase | PAAG_02011 | 2057.00 | 0.482 | |
Phosphoglycerate kinase | PAAG_02869 | 3428.25 | 0.619 | |
Pyruvate kinase | PAAG_06380 | 9829.55 | 0.657 | |
Enolase | PAAG_00771 | 39472.25 | 0.779 | |
Phosphofructokinase subunit | PAAG_01583 | 587.46 | * | |
Pyruvate dehydrogenase E1 component beta subunit | PAAG_01534 | 2794.88 | 0.733 | |
Glyoxylate cycle | Isocitrate lyase | PAAG_04549 | 923.71 | 0.827 |
Krebs cycle | Malate dehydrogenase | PAAG_00053 | 47991.24 | 0.795 |
Malate dehydrogenase | PAAG_08449 | 7490.87 | 0.756 | |
Isocitrate dehydrogenase subunit 1 | PAAG_00856 | 1820.37 | * | |
Isocitrate dehydrogenase subunit 2 | PAAG_07729 | 1604.29 | * | |
Succinate dehydrogenase flavoprotein subunit, mitochondrial | PAAG_01725 | 1798.19 | 0.827 | |
Oxidation of fatty acids | Short-chain specific acyl-CoA dehydrogenase | PAAG_05454 | 1028.15 | * |
Transport | Carbonic anhydrase | PAAG_05716 | 854.25 | 0.795 |
Clathrin light chain | PAAG_08252 | 1049.51 | 0.741 | |
GTP-binding nuclear protein ran-1 | PAAG_04651 | 3676.19 | 0.527 | |
Nipsnap family protein | PAAG_05960 | 4593.91 | 0.677 | |
Vesicular-fusion protein sec17 | PAAG_06233 | 559.70 | * | |
Rab GDP-dissociation inhibitor | PAAG_06344 | 1958.40 | 0.625 | |
Protein fate | G-protein comlpex beta subunit CpcB | PAAG_06996 | 2600.70 | 0.741 |
Protein disulfide-isomerase | PAAG_00986 | 14896.18 | 0.670 | |
Miscellaneous | Thiol-specific antioxidant | PAAG_03216 | 4271.92 | 0.427 |
Translation | Cytosolic large ribosomal subunit protein L30 | PAAG_01050 | 6746.86 | 0.756 |
40S ribosomal protein S0 | PAAG_02111 | 10467.63 | 0.741 | |
40S ribosomal protein S11 | PAAG_06367 | 3129.84 | 0.512 | |
40S ribosomal protein S14 | PAAG_01433 | 1642.34 | 0.712 | |
40s ribosomal protein s15 | PAAG_04690 | 6547.01 | 0.625 | |
40s ribosomal protein s26 | PAAG_07847 | 9205.88 | 0.477 | |
40S ribosomal protein S5 | PAAG_05484 | 5524.47 | 0.625 | |
40S ribosomal protein S7 | PAAG_07182 | 7212.75 | 0.670 | |
40S ribosomal protein S8 | PAAG_00264 | 3915.07 | 0.651 | |
40S ribosomal protein S9 | PAAG_01435 | 2407.07 | 0.487 | |
40S ribosomal protein S9 | PAAG_03828 | 2402.26 | 0.502 | |
60S ribosomal protein L13 | PAAG_06320 | 5338.78 | 0.589 | |
60S ribosomal protein L15 | PAAG_00969 | 4623.56 | 0.468 | |
60S ribosomal protein L18A | PAAG_00952 | 3245.52 | 0.571 | |
60S ribosomal protein L2 | PAAG_00430 | 2292.83 | 0.517 | |
60S ribosomal protein L43 | PAAG_06569 | 12650.10 | 0.543 | |
60S ribosomal protein L4-A | PAAG_08888 | 5405.80 | 0.619 | |
60S ribosomal protein L5 | PAAG_00548 | 911.54 | 0.795 | |
60S ribosomal protein L7 | PAAG_06487 | 3961.73 | 0.748 | |
60S ribosomal protein | PAAG_01834 | 4195.17 | 0.538 | |
60S ribosomal protein L31E | PAAG_04965 | 2514.61 | * | |
Ribosomal protein S23 | PAAG_00385 | 923.83 | * | |
Elongation factor 1-alpha | PAAG_02024 | 13081.64 | 0.284 | |
Elongation factor 1-beta | PAAG_03028 | 26825.63 | 0.427 | |
Elongation factor 1-gamma | PAAG_03556 | 9096.78 | 0.317 | |
Elongation factor 2 | PAAG_00594 | 11304.17 | 0.403 | |
Polyadenylate-binding protein | PAAG_00244 | 1647.87 | 0.631 | |
Ribosomal protein L19 | PAAG_08497 | 3909.55 | 0.607 | |
Ribosomal protein P0 | PAAG_00801 | 2669.74 | 0.560 | |
Ribosomal protein S20 | PAAG_03322 | 1872.13 | 0.763 | |
Ribosomal protein S6 | PAAG_02634 | 1918.03 | 0.589 | |
40S Ribosomal protein S3 | PAAG_01785 | 6921.45 | 0.595 | |
U5 small nuclear ribonucleoprotein component | PAAG_07785 | 372.29 | 0.242 | |
Unclassified | Hypothetical protein | PAAG_07955 | 2234.10 | 0.507 |
Uncharacterized protein | PAAG_07989 | 907.76 | 0.657 | |
Uncharacterized protein | PAAG_04274 | 841.04 | 0.726 | |
Uncharacterized protein | PAAG_02434 | 1488.40 | * | |
Uncharacterized protein | PAAG_07841 | 11750.10 | 0.458 | |
Uncharacterized protein | PAAG_00724 | 3895.92 | 0.492 |
Functional category—based on the MIPS Functional categories database and GO.
Accession number—accession number of matched protein from Paracoccidioides database (http://www.broadinstitute.org/annotation/genome/paracoccidioides_brasiliensis/MultiHome.html).
Proteins detected only in control conditions.