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Cellular uptake and in vitro gene silencing effects of RNV. A) Fluorescence images of MCF-7/ADR cells incubated with free Dox and RNV[Dox/siMDR1]. Scale bar, 200 μm. The fluorescence signal is from Dox. B) 2 h cellular uptake of free Dox and RNV[Dox/siMDR1]. C) 72 h gene silencing experiment measured by RT-qPCR. *P<0.05, **P<0.01, siNC: negative control siRNA. Lipofectamine[siMDR1]+Dox: Lipofectamine2000 loaded with siMDR1 plus free Dox.
