Table III.
Stratified analysis of the association between folate-related gene polymorphisms and colorectal cancer risk.
| Variables | Models | MTHFR 677C>T AOR (95% CI)a | P-value | MTHFR 1298A>C AOR (95% CI)a | P-value | TSER 2R/3R AOR (95% CI)a | P-value | TS 1494 0bp/6bp AOR (95% CI)a | P-value | RFC1 −43T>C AOR (95% CI)a | P-value | RFC1 80G>A AOR (95% CI)a | P-value | RFC1 696C>T AOR (95% CI)a | P-value |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Age, years | |||||||||||||||
| ≤62 | D | 0.88 (0.59–1.32) | 0.545 | 1.05 (0.69–1.61) | 0.809 | 0.76 (0.51–1.14) | 0.187 | 0.69 (0.47–1.00) | 0.052 | 0.75 (0.47–1.21) | 0.239 | 0.73 (0.45–1.17) | 0.191 | 0.74 (0.46–1.18) | 0.204 |
| R | 1.15 (0.67–1.97) | 0.623 | 1.67 (0.39–7.21) | 0.495 | 1.43 (0.31–6.58) | 0.647 | 0.72 (0.37–1.43) | 0.349 | 1.27 (0.85–1.92) | 0.249 | 1.19 (0.80–1.77) | 0.397 | 1.07 (0.71–1.62) | 0.734 | |
| >62 | D | 1.32 (0.89–1.96) | 0.170 | 1.05 (0.70–1.58) | 0.802 | 1.63 (1.09–2.43) | 0.017 | 1.04 (0.72–1.50) | 0.837 | 1.41 (0.89–2.21) | 0.141 | 1.55 (0.97–2.47) | 0.066 | 1.50 (0.96–2.35) | 0.076 |
| R | 0.83 (0.50–1.38) | 0.482 | 3.65 (1.04–12.73) | 0.043 | 1.34 (0.46–3.96) | 0.591 | 1.71 (0.90–3.25) | 0.101 | 1.30 (0.86–1.96) | 0.207 | 0.97 (0.65–1.44) | 0.876 | 1.14 (0.76–1.73) | 0.527 | |
| Gender | |||||||||||||||
| Male | D | 1.02 (0.70–1.48) | 0.929 | 1.21 (0.81–1.80) | 0.347 | 1.03 (0.70–1.49) | 0.895 | 0.84 (0.59–1.19) | 0.321 | 1.01 (0.67–1.52) | 0.976 | 0.99 (0.65–1.50) | 0.946 | 0.97 (0.65–1.46) | 0.888 |
| R | 0.78 (0.47–1.29) | 0.334 | 1.39 (0.37–5.20) | 0.624 | 0.98 (0.32–3.04) | 0.972 | 1.26 (0.69–2.32) | 0.449 | 1.35 (0.92–1.98) | 0.129 | 1.14 (0.78–1.65) | 0.499 | 1.16 (0.78–1.71) | 0.460 | |
| Female | D | 1.03 (0.68–1.55) | 0.900 | 0.89 (0.58-.36) | 0.588 | 1.21 (0.80–1.85) | 0.369 | 0.83 (0.56–1.23) | 0.358 | 1.05 (0.63–1.75) | 0.861 | 1.12 (0.66–1.90) | 0.677 | 1.16 (0.70–1.93) | 0.560 |
| R | 1.18 (0.69–2.02) | 0.545 | 4.62 (1.20–17.80) | 0.026 | 2.18 (0.59–8.08) | 0.242 | 1.02 (0.51–2.07) | 0.949 | 1.26 (0.82–1.94) | 0.287 | 1.03 (0.68–1.56) | 0.886 | 1.07 (0.69–1.64) | 0.772 | |
| HTN | |||||||||||||||
| No | D | 0.90 (0.60–1.36) | 0.623 | 0.76 (0.49–1.17) | 0.208 | 1.19 (0.78–1.81) | 0.424 | 1.01 (0.68–1.50) | 0.972 | 0.88 (0.55–1.41) | 0.603 | 0.79 (0.49–1.27) | 0.329 | 0.90 (0.56–1.43) | 0.653 |
| R | 0.67 (0.37–1.23) | 0.201 | 1.67 (0.48–5.80) | 0.416 | 7.36 (1.44–37.58) | 0.016 | 1.65 (0.84–3.28) | 0.149 | 1.49 (0.96–2.30) | 0.076 | 1.28 (0.84–1.95) | 0.260 | 1.26 (0.80–1.97) | 0.320 | |
| Yes | D | 1.09 (0.75–1.59) | 0.660 | 1.30 (0.88–1.94) | 0.190 | 1.04 (0.72–1.52) | 0.821 | 0.76 (0.53–1.07) | 0.116 | 1.05 (0.68–1.63) | 0.815 | 1.24 (0.79–1.93) | 0.347 | 1.15 (0.75–1.77) | 0.524 |
| R | 1.07 (0.66–1.72) | 0.787 | 3.63 (0.76–17.36) | 0.106 | 0.65 (0.22–1.96) | 0.444 | 0.88 (0.48–1.63) | 0.691 | 1.10 (0.76–1.60) | 0.616 | 0.88 (0.61–1.27) | 0.504 | 0.97 (0.67–1.41) | 0.878 | |
| DM | |||||||||||||||
| No | D | 0.93 (0.68–1.27) | 0.627 | 1.03 (0.74–1.42) | 0.867 | 1.21 (0.88–1.66) | 0.248 | 0.81 (0.60–1.09) | 0.156 | 0.94 (0.65–1.35) | 0.730 | 0.94 (0.65–1.36) | 0.738 | 0.96 (0.66–1.37) | 0.804 |
| R | 0.92 (0.60–1.40) | 0.687 | 2.00 (0.68–5.92) | 0.211 | 1.65 (0.67–4.11) | 0.279 | 1.02 (0.62–1.69) | 0.943 | 1.46 (1.06–2.02) | 0.020 | 1.14 (0.84–1.56) | 0.406 | 1.24 (0.90–1.72) | 0.188 | |
| Yes | D | 1.19 (0.68–2.11) | 0.540 | 1.01 (0.56–1.82) | 0.976 | 0.90 (0.51–1.57) | 0.699 | 1.05 (0.61–1.82) | 0.863 | 1.23 (0.65–2.34) | 0.530 | 1.24 (0.64–2.39) | 0.525 | 1.27 (0.68–2.38) | 0.452 |
| R | 0.93 (0.4–1.93) | 0.841 | 3.76 (0.46–30.92) | 0.218 | 0.84 (0.07–9.67) | 0.891 | 2.34 (0.65–8.43) | 0.194 | 0.84 (0.47–1.50) | 0.548 | 0.84 (0.47–1.49) | 0.555 | 0.75 (0.41–1.35) | 0.330 | |
| Tumor site | |||||||||||||||
| Colon | D | 0.98 (0.71–1.36) | 0.915 | 0.90 (0.64–1.26) | 0.526 | 1.05 (0.76–1.46) | 0.757 | 0.78 (0.58–1.06) | 0.117 | 1.21 (0.82–1.78) | 0.331 | 1.17 (0.79–1.73) | 0.432 | 1.19 (0.82–1.75) | 0.361 |
| R | 0.90 (0.58–1.39) | 0.631 | 2.01 (0.69–5.82) | 0.198 | 0.95 (0.31–2.92) | 0.932 | 1.21 (0.72–2.04) | 0.479 | 1.36 (0.98–1.89) | 0.065 | 1.08 (0.79–1.50) | 0.620 | 1.17 (0.84–1.63) | 0.359 | |
| Rectum | D | 1.02 (0.71–1.46) | 0.922 | 1.25 (0.86–1.80) | 0.238 | 1.15 (0.80–1.65) | 0.440 | 0.97 (0.69–1.36) | 0.876 | 0.80 (0.54–1.20) | 0.297 | 0.86 (0.57–1.30) | 0.476 | 0.87 (0.58–1.29) | 0.489 |
| R | 1.01 (0.63–1.60) | 0.982 | 3.27 (1.13–9.47) | 0.029 | 1.97 (0.72–5.38) | 0.186 | 1.14 (0.63–2.05) | 0.665 | 1.11 (0.77–1.61) | 0.578 | 0.97 (0.68–1.39) | 0.879 | 0.93 (0.64–1.36) | 0.702 | |
| Tumor size | |||||||||||||||
| < 5cm | D | 1.20 (0.83–1.73) | 0.338 | 0.76 (0.51–1.12) | 0.163 | 1.01 (0.70–1.45) | 0.978 | 0.83 (0.59–1.17) | 0.296 | 0.99 (0.65–1.49) | 0.947 | 0.95 (0.63–1.44) | 0.804 | 1.04 (0.69–1.56) | 0.864 |
| R | 1.25 (0.80–1.95) | 0.337 | 1.17 (0.32–4.29) | 0.816 | 1.59 (0.55–4.59) | 0.393 | 0.66 (0.33–1.33) | 0.247 | 1.00 (0.69–1.46) | 0.989 | 0.89 (0.62–1.29) | 0.544 | 0.90 (0.62–1.33) | 0.609 | |
| ≥ 5cm | D | 0.86 (0.63–1.19) | 0.370 | 1.28 (0.92–1.77) | 0.141 | 1.17 (0.85–1.61) | 0.348 | 0.86 (0.63–1.16) | 0.311 | 1.00 (0.69–1.46) | 0.984 | 1.08 (0.73–1.58) | 0.709 | 0.99 (0.69–1.42) | 0.948 |
| R | 0.74 (0.47–1.16) | 0.189 | 3.63 (1.38–9.53) | 0.009 | 1.40 (0.52–3.81) | 0.509 | 1.57 (0.96–2.56) | 0.072 | 1.44 (1.05–1.99) | 0.026 | 1.14 (0.83–1.57) | 0.412 | 1.18 (0.85–1.64) | 0.315 | |
| L/N invasion | |||||||||||||||
| No | D | 0.82 (0.59–1.14) | 0.248 | 1.08 (0.77–1.52) | 0.665 | 1.14 (0.81–1.58) | 0.457 | 0.88 (0.65–1.21) | 0.436 | 0.77 (0.53–1.11) | 0.162 | 0.88 (0.60–1.28) | 0.496 | 0.77 (0.53–1.11) | 0.161 |
| R | 1.00 (0.65–1.54) | 0.994 | 2.32 (0.80–6.77) | 0.123 | 2.81 (1.17–6.76) | 0.021 | 1.00 (0.57–1.77) | 0.994 | 1.04 (0.73–1.47) | 0.838 | 0.81 (0.58–1.14) | 0.237 | 0.88 (0.61–1.25) | 0.466 | |
| Yes | D | 1.23 (0.87–1.75) | 0.249 | 1.00 (0.70–1.43) | 1.000 | 1.05 (0.75–1.49) | 0.766 | 0.80 (0.58–1.10) | 0.173 | 1.39 (0.91–2.11) | 0.129 | 1.22 (0.80–1.84) | 0.354 | 1.42 (0.94–2.16) | 0.100 |
| R | 0.89 (0.56–1.40) | 0.606 | 2.71 (0.94–7.76) | 0.064 | 0.21 (0.03–1.65) | 0.137 | 1.35 (0.79–2.32) | 0.271 | 1.52 (1.08–2.14) | 0.017 | 1.33 (0.95–1.85) | 0.097 | 1.30 (0.92–1.84) | 0.141 | |
a
Models adjusted for age, gender, hypertension and diabetes mellitus status. MTHFR, methylenetetrahydrofolate reductase; TS, thymidylate synthase; TSER, TS enhancer region; RFC1, reduced folate carrier 1; AOR, adjusted odds ratio; CI, confidence interval; D, dominant; R, recessive; HTN; hypertension; DM, diabetes mellitus; L/N, lymph node.