Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Enantioselectivity of wild-type CalA and the Y93L/L367I variant determined for the kinetic resolution of various secondary alcohols by transacylation in isooctane at 21 °C.[a]
| Entry | Alcohol | Ewild-type | EY93L/L367I |
|---|---|---|---|
| 1 |  |
3 | 100 |
| 2 |  |
5 | 20 |
| 3 |  |
30 | >300 |
| 4 |  |
70 | >300 |
| 5 |  |
70 | 100 |
| 6 |  |
32 | 23 |
| 7 |  |
4 | 100 |
Reaction conditions: 200 mm vinyl butyrate, 20 mm alcohol, and 20 mm dodecane in isooctane at 21 °C. The enzyme was immobilized on Nunc 96-well nickel-coated microtiter plates.
