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Enantioselectivity of CalA single mutants determined for the kinetic resolution of 1-phenylethanol (model reaction).[a]
| CalA variant | ees [%] | eep [%] | Conversion [%] | E |
|---|---|---|---|---|
| Y93L | 62 | 86 | 44 | 25 |
| L367I | 12 | 86 | 25 | 4 |
Reaction conditions: 200 mm vinyl butyrate, 20 mm 1-phenylethanol, 20 mm dodecane in isooctane at 21 °C. The enzyme was immobilized on 96-well nickel-coated microtiter plates.
